Topics: Origin: Project type: Development
Name of Applicant: Marianne De Paepe
Date of application: 01-09-2025
Unit: Other
Location: Phone: 0664803737
@ Mail: marianne.depaepe@inrae.fr
@ PI-Mail: philippe.noirot@inrae.fr
Project context and summary: The mutation rate of phage T4 has been traditionally measured through fluctuation assays, by detecting mutations in specific genes confering an identiable phenotype. Yet such method is altogether not very accurate, cumbersome, and suffer from many bias. In this project we aim at determining the mutation rate of phage T4 by whole genome sequencing of a large number of individual plaques. This would provide the first genomic estimate of T4 phage mutation rate.
Related team publications:Atypical Replication Error Pattern and Limited Repair Efficiency Contribute to Elevated Mutation Rate in Phage lambda Julien Lopez, Magali Ventroux, Valentin Loux, Mahendra Mariadassou, Francois Lecointe, Rafael Sanjuan, Marina Elez, Marianne De Paepe. bioRxiv 2025.08.29.673000; doi: https://doi.org/10.1101/2025.08.29.673000
Status: New
