Project

Go Back to Project List
#18967 : Comparing neutrophil responses from ITP patients and healthy donors
Topics: Transcriptomics (Illumina)
Origin: IP
Project type: Expertise

Name of Applicant: Friederike Jönsson
Date of application: 06-11-2024
Unit: Antibodies in Therapy and Pathology
Location: 64-04-44
Phone: +33 (0)1 53 55 12 65
@ Mail: friederike.jonsson@pasteur.fr

Project context and summary:

Immune thrombocytopenia (ITP) is an autoimmune disease characterized by autoantibodies targeting platelets resulting in a low platelet count and an increased risk of bleeding. Platelet phagocytosis is mediated by antibody platelet opsonization and complement activation. Although neutrophils are professional phagocytes and are functionally equipped to act at different steps of ITP, their contribution to the disease remains understudied. Neutrophils express complement and Fc receptors and can phagocytose platelets. Moreover, neutrophils accumulate in the spleen of ITP patients where they settle in proximity to a specific B cell subset. Previous studies have suggested a role for neutrophils in the regulation of adaptive immunity especially in B cell activation and proliferation: In the spleen, “B helper neutrophils” can produce cytokines such as interleukin-21 (IL-21), B-cell activating factor (BAFF) and a proliferation-inducing ligand (APRIL) that induce B cell maturation into antibody-secreting plasma cells. These elements strongly support the implication of neutrophils in ITP. The objective of our project is to identify differentially expressed genes in neutrophils from ITP patients versus healthy donors to better understand their implication in the pathophysiology of ITP.


Related team publications:
Krémer V, de Chaisemartin L, Jönsson F. The role of neutrophils in antibody-driven autoimmune cytopenias. Int J Biochem Cell Biol. 2022 Jun;147:106231. doi: 10.1016/j.biocel.2022.106231. Epub 2022 May 26. PMID: 35644471.
Jönsson F, de Chaisemartin L, Granger V, Gouel-Chéron A, Gillis CM, Zhu Q, Dib F, Nicaise-Roland P, Ganneau C, Hurtado-Nedelec M, Paugam-Burtz C, Necib S, Keita-Meyer H, Le Dorze M, Cholley B, Langeron O, Jacob L, Plaud B, Fischler M, Sauvan C, Guinnepain MT, Montravers P, Aubier M, Bay S, Neukirch C, Tubach F, Longrois D, Chollet-Martin S, Bruhns P. An IgG-induced neutrophil activation pathway contributes to human drug-induced anaphylaxis. Sci Transl Med. 2019 Jul 10;11(500):eaat1479. doi: 10.1126/scitranslmed.aat1479. PMID: 31292264.
Service Delivery
Manager: iakov.vitrenko@pasteur.fr
Status: Libraries


Go Back to Project List