The project titled “Antimicrobial Resistance Profile and Molecular Epidemiological Analysis of Extended Spectrum Β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Clinical Specimens” aims to investigate the prevalence and characteristics of Enterobacteriaceae isolates that produce extended-spectrum β-lactamases (ESBLs) and carbapenemases. These enzymes confer resistance to a broad range of antibiotics, posing a significant challenge to clinicians. The project will involve analyzing clinical specimens from patients with bacterial infections to determine the prevalence of ESBL- and carbapenemase-producing Enterobacteriaceae and their antibiotic susceptibility profiles.
To achieve this, the project will employ the polymerase chain reaction (PCR) and sequencing techniques. PCR is a laboratory technique used to rapidly produce millions to billions of copies of a specific segment of DNA
. In this project, PCR will be used to amplify the target DNA regions associated with ESBL and carbapenemase production in Enterobacteriaceae isolates
. The amplified DNA will then undergo sequencing, which involves determining the exact nucleotide sequence of the DNA segment.
By utilizing PCR and sequencing, the project aims to gain insights into the antimicrobial resistance profile and molecular epidemiology of ESBL- and carbapenemase-producing Enterobacteriaceae isolates from clinical specimens. The findings from this research will contribute to a better understanding of these resistant bacteria and guide the development of effective antimicrobial strategies.
