In collaboration with the CIGM, we have generated a new knock-in mouse model by using CRISPR-CAS9 + a repair DNA matrix.
We would like to know if CRISPR-CAS9 have cut in an unexpected site and if the DNA matrix has been inserted in that sites in some of these mice.
The ultimated goal is to select 1 founder whithout any off-site cleavage to initiate our mouse colony.
