Topics: Transcriptomics (Illumina)
Origin: IP
Project type: Expertise
Name of Applicant: Marc Lavigne
Date of application: 18-12-2023
Unit: Department of Virology
Location: Lwoff 2nd fllor
Phone: 0
@ Mail: marc.lavigne@pasteur.fr
Project context and summary: G4 structures are non-canonical nucleic acid structures regulating gene expression and chromatin organization. These structures also regulate the replication of several viruses such as HIV-1. The aims of this project are to identify specific G4 ligands interacting with G4s present in HIV-1 LTR promoter and to study their effects on viral expression and chromatin organization surrounding the integrated viral genome. For this purpose, we will perform both transcriptomic and chromatin conformation capture studies in cell lines containing a silent and reactivable integrated HIV-1 genome, in the presence and absence of selected G4-ligands. These studies should reveal new mechanisms regulating HIV-1 expression that could be used as antiviral targets.
Related team publications:Lavigne M*, Helynck O, Rigolet P, Nowakowski M, Brûlé S, Hoos S, Raynal B, Guittat L, Beauvineau C, Petres S, Granzhan A, Guillon J,, Pratviel G, Teulade-Fichou MP, England P*, Mergny JL*,Munier-Lehmann H*. SARS-CoV-2 Nsp3 Unique Domain SUD interacts with guanine quadruplexes and G4-ligands inhibit this interaction. Nucleic Acids Res., 49, 7695-7712
Lista M.J., Jousset A.C., Cheng M., Saint-André V., Gadelle D., Toccafondi E., Juchet A., Segeral E., Berlioz-Torrent C., Emiliani S., Mergny J.L., Lavigne M*. DNA topoisomerase 1 represses HIV-1 basal transcription through its interaction with a guanine quadruplex present in the viral promoter. Retrovirology, 2023; 20:10
A, Lavigne M, Rezaei M., Nikzad S, Zare P, Mergny JL *, Rahimi H-R. Unlocking G-quadruplexes as antiviral targets. Phamacological Reviews, 2021, 73(3):897-923.
Manager: elodie.turc@pasteur.frStatus: Data analysis
