Project

Go Back to Project List
#17439 : African Cell Atlas
Topics: Single Cells
Origin: IP
Project type: Service

Name of Applicant: Maxime ROTIVAL
Date of application: 02-10-2023
Unit: Human Evolutionary Genetics
Location: Jacob – 5th floor – office 26.05.06A
Phone: 0140613634
@ Mail: maxime.rotival@pasteur.fr
@ PI-Mail: quintana@pasteur.fr

Project context and summary:

Project context and summary:

The project aims (i) to identify the genetic and GxE determinants driving variation in gene expression and chromatin accessibility in African ancestry individuals, and (ii) to characterize the transcriptomic & epigentic variation of immune cells in populations of African descnt in respone to immune stimulation (using LPS as a proxy for bacterial infection), comparing across urban and rural settings, and differnt sex and age groups.

To do so, the project will build on a subset of 400 donors from 8 populations, to which we are contributiong one specific population (48 central Africans, recruited in Belgium). Epigenetic (snATAC-seq) & transcriptomic (snRNA-seq) landscape of peripheral blood mononuclear cells from these donors will be characterized after 6h of stimulation with LPS and contrasted with profules of unstimulated cells (Null).


Related team publications:
Aquino, Y., Bisiaux, A., Li, Z. et al. Dissecting human population variation in single-cell responses to SARS-CoV-2. Nature 621, 120–128 (2023). https://doi.org/10.1038/s41586-023-06422-9
Service Delivery
Manager: marc.monot@pasteur.fr
Status: Closed


Go Back to Project List