Topics: Transcriptomics (Illumina)
Origin: IP
Project type: Expertise
Name of Applicant: Nadia Benaroudj
Date of application: 22-08-2023
Unit: Biology of Spirochetes
Location: 12-03-16
Phone: 0140613746
@ Mail: nadia.benaroudj@pasteur.fr
@ PI-Mail: mathieu.picardeau@pasteur.fr
Project context and summary: Pathogen leptospires are responsible for the zoonotic disease leptospirosis. The clinical manifestations of this infection range from a febrile state to a severe life-threatening form characterized by multiple organ hemorrhages. More than one million cases of leptospirosis are currently reported annually in the word, with 10% of mortality.
Leptospira are extracellular pathogens that penetrate hosts and rapidly disseminate to target organs (including kidney, liver, lungs) throughout the bloodstream. During infection, Leptospira are confronted with deadly oxidants, including superoxide radical. We have isolated a L. interrogans strain that has acquired a steady resistance to superoxide. Our aim is to identify gene expression-based mechanism underlying the resistance to superoxide.
Related team publications:Zavala-Alvarado C, Sismeiro O, Legendre R, Varet H, Bussotti G, Bayram J, G Huete S, Rey G, Coppée JY, Picardeau M, Benaroudj N. (2020) The transcriptional response of pathogenic Leptospira to peroxide reveals new defenses against infection-related oxidative stress. PLoS Pathog. Oct;16(10):e1008904 DOI: 10.1371/journal.ppat.1008904
Zavala-Alvarado C, G Huete S, Vincent AT, Sismeiro O, Legendre R, Varet H, Bussotti G, Lorioux C, Lechat P, Coppée JY, Veyrier FJ, Picardeau M, Benaroudj N. (2021) The oxidative stress response of pathogenic Leptospira is controlled by two peroxide stress regulators which putatively cooperate in controlling virulence. PLoS Pathog. Dec 2;17(12):e1009087. DOI: 10.1371/journal.ppat.1009087
G Huete S, Benaroudj N. (2023) The arsenal of Leptospira species against oxidants. Antioxidants 2023, 12(6), 1273; https://doi.org/10.3390/antiox12061273
Manager: thomas.cokelaer@pasteur.frStatus: Closed
