Project

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#17009 : Milieu Intérieur scRNAS-seq : BATCH1
Topics: Single Cells
Origin: IP
Project type: Expertise

Name of Applicant: Maxime ROTIVAL
Date of application: 07-07-2023
Unit: Human Evolutionary Genetics
Location: Jacob – 5th floor – office 26.05.06A
Phone: 0140613634
@ Mail: maxime.rotival@pasteur.fr
@ PI-Mail: quintana@pasteur.fr

Project context and summary:

The project aims to characterize the effect of ageing on human immune responses to respiratory RNA viruses (Influenza A virus & SARS-CoV-2) in the Milieu Intérieur cohort and characterize the genetic, epigenetic & environmental factors underlying variability of antiviral responses.

To do so, the project will build on a subset of 400 donors from the Milieu Intérieur cohort, aged 30-80 years old at the time of sample collection (balanced males/females ratio). Epigenetic landscape of peripheral blood mononuclear cells from these donors will be characterized at basal state (snATAC-seq, 1850 cells per donor), and contrasted with response to viruses measured 6h after infection (scRNAseq, 1650 cells per donor x 3 conditions: Null, IAV and SARS-CoV-2)

The cells will be processed in two spearate batches. the first batch will include 172 donors across 88 scRNA-seq and 33 snATAC-seq libraires.


Related team publications:
Aquino et al, Environmental and genetic drivers of population differences in SARS-CoV-2 immune responses, BiorXiv, 2022, doi: https://doi.org/10.1101/2022.11.22.517073
Piasecka et al, Distinctive roles of age, sex, and genetics in shaping transcriptional variation of human immune responses to microbial challenges, PNAS,2018 Jan; 115(3): E488-E497, doi: https://doi.org/10.1073/pnas.1714765115
Service Delivery
Manager: marc.monot@pasteur.fr
Status: Closed


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