The project aims to characterize the effect of ageing on human immune responses to respiratory RNA viruses (Influenza A virus & SARS-CoV-2) in the Milieu Intérieur cohort and characterize the genetic, epigenetic & environmental factors underlying variability of antiviral responses.
To do so, the project will build on a subset of 400 donors from the Milieu Intérieur cohort, aged 30-80 years old at the time of sample collection (balanced males/females ratio). Epigenetic landscape of peripheral blood mononuclear cells from these donors will be characterized at basal state (snATAC-seq, 1850 cells per donor), and contrasted with response to viruses measured 6h after infection (scRNAseq, 1650 cells per donor x 3 conditions: Null, IAV and SARS-CoV-2)
The cells will be processed in two spearate batches. the first batch will include 172 donors across 88 scRNA-seq and 33 snATAC-seq libraires.
