Topics: Transcriptomics (Illumina)
Origin: Academic
Project type: Service
Name of Applicant: Ciarán Condon
Date of application: 29-03-2022
Unit: Other
Location: Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75013 Paris
Phone: 0698009627
@ Mail: condon@ibpc.fr
Project context and summary: We recently identified a new RNA decapping enzyme in Bacillus subtilis, called BsRppH2, with a preference for mRNAs beginning with an A-residue. The goal of this project is to identify individual RNAs whose abundance is correlated with the presence or absence of BsRppH2, to get an idea of its global importance in vivo. We will sequence RNA in triplicate from 4 strains: WT, ∆rppH2, a complemented strain (∆rppH2 + RppH2) and WT + RppH2.
Related team publications:Piton, J., Larue, V., Thillier, Y., Dorléans, A., Pellegrini, O., Li de la Sierra-Gallay, I., Vasseur, J.J., Debart, F., Tisné, C. and Condon, C. (2013) The B. subtilis RNA deprotection enzyme RppH recognizes guanosine in the second position of its substrates. PNAS (110) 8858-8863.
Richards, J, Liu, Q, Pellegrini, O, Celesnik, H, Yao, S, Bechhofer, DH, Condon, C, and Belasco, JG. (2011) An RNA pyrophosphohydrolase triggers 5’-exonucleolytic degradation of mRNA in Bacillus subtilis. Mol. Cell. 43:940-949.
Manager: marc.monot@pasteur.frStatus: Closed
