Topics: Genomics (Illumina)
Origin: IP
Project type: Service
Name of Applicant: Virginija Cvirkaite-Krupovic
Date of application: 09-03-2022
Unit: Other
Location: 68-03-39
Phone: 8819
@ Mail: virginija.cvirkaite-krupovic@pasteur.fr
@ PI-Mail: mart.krupovic@pasteur.fr
Project context and summary: We are studying the diversity of viruses infecting hyperthermophilic archaea. We have collected environmental samples from the hot springs in Japan, Costa Rica and Chile. The samples were used to start the enrichment cultures. After 10 days of incubation the samples were checked under TEM. Viruses with different virion morphologies were observed, including lemon-shaped, rod-shaped, filamentous, spiral-like and spherical.
Our objectibve is to sequence the total viral DNA extracted from the supernatants of the enrichment cultures and also from the pure, isolated viruses. The genomes of the archaeal viruses are usually dsDNA and of 15-70kb in size.
Related team publications:Diana P Baquero, Patrizia Contursi, Monica Piochi, Simonetta Bartolucci, Ying Liu, Virginija Cvirkaite-Krupovic, David Prangishvili and Mart Krupovic. New virus isolates from Italian hydrothermal environments underscore the biogeographic pattern in archaeal virus communities. ISME J . 2020 Jul;14(7):1821-1833 PMID: 32322010
Liu Y, Brandt D, Ishino S, Ishino Y, Koonin EV, Kalinowski J, Krupovic M and Prangishvili D. New archaeal viruses discovered by metagenomic analysis of viral communities in enrichment cultures. Environ Microbiol . 2019 Jun;21(6):2002-2014.PMID: 30451355
iu Y, Ishino S, Ishino Y, Pehau-Arnaudet G, Krupovic M, Prangishvili D. A Novel Type of Polyhedral Viruses Infecting Hyperthermophilic ArchaeaJ Virol. 2017 Jun 9;91(13):e00589-17. PMID: 28424284
Manager: chloe.baum@pasteur.frStatus: Closed
