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#6781 : Metagenomics analysis of hospital effluents as a tool for monitoring antimicrobial consumption in the context of the COVID-19 pandemic in Cameroon
Topics: Metagenomics (Shotgun)
Origin: RIIP
Project type: Service

Name of Applicant: Ariane Nzouankeu
Date of application: 16-02-2022
Unit: Other
Location: Yaoundé
Phone: +237677622041
@ Mail: nzouankeu@pasteur-yaounde.org

Project context and summary:

COVID-19 arose at the time of great concern about Antimicrobial resistance (AMR). Despite the fact that little evidence on the impact of antimicrobials use for the treatment of COVID-19 is available, infection prevention and control policy include use of antimicrobials. The increasing use of antimicrobials and biocides for COVID-19 treatment or environmental disinfection may result in high proportion of these compounds in surrounding wastewater, and increase in AMR. We believe that Hospital wastewater contents in residual antimicrobial compounds and antimicrobial resistance determinants s may correlate with their levels of antimicrobial consumption in Cameroon during the COVID-19 outbreak.
Hospitals effluents will be collected in the two cities recording the highest numbers of COVID-19 cases in Cameroon. In each, 02 healthcare facilities receiving COVID-19 patients for treatment and 02 not involved in COVID-19 management will be selected for the study. Wastewater samples will be screened for detection and quantification of AMR genetic determinants using metagenomics analysis. The diversity and load of antimicrobials residues in the samples will be evaluated using Liquid Chromatography coupled with Mass Spectrometry. Based on the magnitude of AMR determinants and antimicrobial residues in the sawages samples, a modelling approach relying on an agglomerative hierarchical clustering analysis will be developed to estimate the antimicrobial consumption level in the selected healthcare facilities. The Bland-Altman analysis coupled to the calculation of the risk quotients will be used to assess the potential environmental and human health risk posed by the released of antimicrobial determinants and residues in surface water bodies. We expect to propose an innovative and non-human related tool for estimating antimicrobial consumption in healthcare facilities using hospital effluents and for providing timely informations on the dissemination of AMR in risk populations.


Related team publications:
1. Serges Tchatchouang, Ariane Nzouankeu, EvaHong, AudeTerrade, Mélanie Denizon, Ala-Eddine Deghmane, Suzie Moyo Tetang Ndiang, Eric-Walter Pefura-Yone, Véronique Penlap Beng, Richard Njouom, Marie-Christine Fonkoua, Muhamed-Kheir Taha. Analysis of Haemophilus species in patients with respiratory tract infections in Yaoundé, Cameroon. International Journal of Infectious Diseases. Volume 100, November 2020, Pages 12-20.
2. T Crucitti , S Belinga, MC Fonkoua, M Abanda, W Mbanzouen, E Sokeng and A Nzouankeu . Sharp increase in ciprofloxacin resistance of Neisseria gonorrhoeae in Yaounde, Cameroon: analyses of a laboratory database period 2012–2018. International Journal of STD & AIDS 2020, Vol. 31(6) 579–586
6. Ariane NZOUANKEU, Marie-Christine FONKOUA, Marguerite WOUAFO, Thomas NJINE, Awa Aidara KANE, Antoinette NGANDJIO. 2017. Molecular characterization of multidrug resistant Salmonella from chicken and humans in Yaounde. Medical Research Archives 4 (8).
Service Delivery
Manager: marc.monot@pasteur.fr
Status: Closed


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