Topics: Transcriptomics (Illumina)
Origin: IP
Project type: Service
Name of Applicant: Alexandre GIRAUD–GATINEAU
Date of application: 10-09-2021
Unit: Biology of Spirochetes
Location: Biotop-3-14
Phone: 0140613406
@ Mail: agiraudg@pasteur.fr
@ PI-Mail: mathieu.picardeau@pasteur.fr
Project context and summary: Pathogen leptospires are responsible for the zoonotic disease leptospirosis. The clinical manifestations of this infection range from a febrile state to a severe life-threatening form characterized by multiple organ hemorrhages and failure. More than one million cases of leptospirosis are currently reported annually in the word, with 10% of mortality.
Pathogen leptospires penetrate host organisms through skin abrasion or mucous membranes, are able to escape innate immunity and rapidly disseminate through the bloodstream to target organs, including liver and kidneys.
The strategies used by pathogenic leptospires for successful host colonization and virulence are not fully understood. In this study, we wanted to understand how pathogenic Leptospira can survive in the bloodstream and to escape serum proteolytic activity.
Related team publications:Gaultney RA, Vincent AT, Lorioux C, Coppée JY, Sismeiro O, Varet H, Legendre R, Cockram CA, Veyrier FJ, Picardeau M. 4-Methylcytosine DNA modification is critical for global epigenetic regulation and virulence in the human pathogen Leptospira interrogans. Nucleic Acids Res. 2020 Dec 2;48(21):12102-12115. doi: 10.1093/nar/gkaa966. PMID: 33301041; PMCID: PMC7708080.
Zavala-Alvarado C, Sismeiro O, Legendre R, Varet H, Bussotti G, Bayram J, G Huete S, Rey G, Coppée JY, Picardeau M, Benaroudj N. The transcriptional response of pathogenic Leptospira to peroxide reveals new defenses against infection-related oxidative stress. PLoS Pathog. 2020 Oct;16(10):e1008904. PubMed PMID: 33021995; PubMed Central PMCID: PMC7567364.
Manager: marc.monot@pasteur.frStatus: Closed
