Topics: Transcriptomics (Illumina)
Origin: Academic
Project type: Service
Name of Applicant: Lise CREMET
Date of application: 08-09-2021
Unit: Bacteria-Cell Interactions
Location: EA3826 IRS2 Nantes Biotech, 22 bd Benoni Goullin, 44200 NANTES
Phone: 0240083957
@ Mail: lise.cremet@chu-nantes.fr
@ PI-Mail: lise.cremet@chu-nantes.fr
Project context and summary: In ventilator-associated pneumonia (VAP), a low relative abundance of Streptococcus spp. within the lung microbiome at the time of intubation has been associated with higher mortality at day 28, and an enrichment with Enterobacteriaceae has been highlighted as an aggravating factor (1,2). It has been recently shown that the use of probiotics would reduce the incidence of VAP, duration of mechanical ventilation, and length of ICU stay (3). Furthermore, a recent study demonstrated that microaspirations of oral microbes would mitigate host susceptibility to infection with lower airway pathogens (4). In that context, we study the in vitro interactions between oral commensal streptococcal species (S. mitis, S. salivarius) and common respiratory pathogens (E. coli and P. aeruginosa) in order to explore antagonistic effects between them.
1. Dickson RP, et al. Am J Respir Crit Care Med. 2020;201(5):555-63.
2. Woo S, et al. J Clin Med. 2020 Feb 27;9(3).
3. Batra P, et al. J Intensive Care. 2020 Oct 15;8(81).
4. Wu BG, et al. Am J Respir Crit Care Med. 2021 May 1;203(9):1099-1111.
Related team publications:Crémet L, Leroy AG, Muller D, Delanou S, Burghelea A, Broquet A, Roquilly A, Caroff N. Antibiotic resistance heterogeneity and LasR diversity within Pseudomonas aeruginosa populations from pneumonia in intensive care unit patients. Int J Antimicrob Agents. 2021 Jun;57(6):106341. doi: 10.1016/j.ijantimicag.2021.106341. Epub 2021 Ap
Crémet L, Gaborit B, Bouras M, Drumel T, Guillotin F, Poulain C, Persyn E, Lakhal K, Rozec B, Vibet MA, Roquilly A, Gibaud S. Evaluation of the FilmArray Pneumonia Plus Panel for rapid diagnosis of Hospital-Acquired Pneumonia in intensive care unit patients. Front Microbiol. 2020 Aug 25;11:2080. doi: 10.3389/fmicb.2020.02080. eCollection 2020
Roquilly A, Torres A, Villadangos JA, Netea MG, Dickson R, Becher B, Asehnoune K. Pathophysiological role of respiratory dysbiosis in hospital-acquired pneumonia. Lancet Respir Med. 2019 Aug;7(8):710-720. doi: 10.1016/S2213-2600(19)30140-7. Epub 2019 Jun 7.
Manager: elodie.turc@pasteur.frStatus: Closed
