Candida albicans is primarily a fungal commensal of the human gastro-intestinal and genital tracts and yet, responsible for superficial or disseminated, often deadly infections. Like many other microorganisms, C. albicans also survives in a community, such as biofilm. From an ORFeome collection composed of 5099 ORF cloned in a GatewayTM donor vector (Legrand et al. 2018), and representing 83% of total annotated coding sequences of C. albicans, we constructed 2451 barcoded doxycycline-inducible over-expression strains. We utilized this collection to identify genes that modulate biofilm formation in C. albicans.
In this study, we identified several genes whose over-expression results in impaired biofilm formation. To understand the mechanism, we are interested in finding the targets of key biofilm regulators emerged from this study by performing ChIP-sequencing.
This study is the continuation of Biomics project #4003.
