CRISPR/Cas9 technology has revolutionized our ability of genome editing and is a very promising tool for gene therapy. However, it has been shown that Cas9-based genome editing can result into extensive on-target damage, including large deletions and complex genomic rearrangements.
Our project aims at exploring the genomic alterations (eg. large deletions, insertions, invertions) at the targeted genomic locus as an outcome of CRISPR/Cas9-based genome editing. We are interested in carrying out targeted long read sequencing of our region of interest, using the PacBio technology and the CRISPR/Cas9-based enrichment procedure. The structural variations at the vicinity of the target site will be analysed in order to evaluate the precision of our genome engineering.
