Topics: Transcriptomics (Illumina)
Origin: Academic
Project type: Service
Name of Applicant: Maude Guillier
Date of application: 25-09-2020
Unit: Other
Location: IBPC, 13 rue Pierre et Marie Curie, 75005 Paris
Phone: 01 58 41 51 49
@ Mail: maude.guillier@ibpc.fr
Project context and summary: Small RNAs are widespread regulators of gene expression. In the model bacterium Escherichia coli, many of them act at the post-transcriptional level and affect the levels of their mRNA targets. Furthermore, sRNAs commonly exist under different isoforms, often through the processing of the primary transcript (possibly a sRNA itself) into a shorter sRNA. In this project, we want to compare the regulons of two small RNAs of E. coli that both exist as a primary transcript or as a processed form devoid of the 5’end of the sRNA.
For this purpose, we will perform a transcriptomic analysis of strains overexpressing either form of each sRNA under the control of an inducible promoter. Overexpression will be performed over a short time (10 minutes) to limit the indirect effects.
Related team publications:Jagodnik J., Chiaruttini C. and Guillier M., 2017, Stem-loop structures within mRNA coding sequences activate translation initiation and mediate control by small regulatory RNA, Molecular Cell, 68(1): 158-170.
Brosse A., Korobeinikova A., Gottesman S. and Guillier M., 2016, Unexpected properties of sRNA promoters allow feedback control via regulation of a two-component system, Nucl. Acids Res., 44(20): 9650-9666.
Brosse A. and Guillier M., 2018, Bacterial small RNAs in mixed regulatory networks, Microbiology Spectrum, 6(3). doi : 10.1128/microbiolspec.RWR-0014-2017.
Manager: valerie.briolat@pasteur.frStatus: Closed
