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#4215 : Analysis of induced point mutation in the multiple copies of a transgenic mouse
Topics: Genomics (Illumina)
Origin: IP
Project type: Expertise

Name of Applicant: Friederike JONSSON
Date of application: 09-07-2020
Unit: Antibodies in Therapy and Pathology
Location: Metchnikoff – 4th floor
Phone: 01-40-61-35-45
@ Mail: joensson@pasteur.fr

Project context and summary:

We used a CRISPR/Cas9 approach to induce a point mutation in a transgenic mouse, carrying approximately 10 copies of a human transgene.
To validate the efficacy of amino acid exchange and the frequency of still functional copies of the transgene we would like to sequence and amplicon of 347 base pairs using Illumina.


Related team publications:
Service Delivery
Manager: marc.monot@pasteur.fr
Status: Closed


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