Candida albicans is primarily a fungal commensal of the human gastro-intestinal and genital tracts and yet, responsible for superficial or disseminated, often deadly infections. Like many other microorganisms, C. albicans also survives in a community lifestyle such as biofilm. From an ORFeome collection composed of 5099 ORF cloned in a GatewayTM donor vector (Legrand et al. 2018), and representing 83% of total annotated coding sequences of C. albicans, we constructed 2451 barcoded doxycycline-inducible over-expression strains. We utilized this collection to identify genes that modulate biofilm development in C. albicans.
In this study, we identified several genes whose over-expression results in impaired biofilm formation. To understand the mechanism, we are interested in transcriptional profiling of key biofilm regulators emerged from this study.
This study is the continuation of Biomics project #3244.
