We want to create a deletion library of H pylori.
This bacteria has a small genome of 1500 genes that will be remove individually by homologous recombination.
Each gene will be replace by a kanamycin cassette.
We want this library to be ordered but also pooled.
To sequence the pooled library and do fitness experiments we need to insert specific sequences during the homologous recombination in order to perform the first PCR for illumina sequencing :
-a TAG specific to each gene deleted (NNNNNN)
-an RBS
-a sequence to perform the first PCR
