Topics: Metagenetics (16s / 18S / ITS)
Origin: IP
Project type: Expertise
Name of Applicant: Anna-Bella FAILLOUX
Date of application: 04-03-2026
Unit: Arboviruses and Insect Vectors
Location: Nicolle – RDC
Phone: 0140613617
@ Mail: anna-bella.failloux@pasteur.fr
Project context and summary: The main objective of the project will be to study the impact of exposure to antibiotics of immature stages of Ae. aegypti on the microbiota of mosquito adults and on the transmission of arboviruses. More specifically, it will involve: (i) measuring the intensity of transfer of antibiotic-resistant Serratia marcescens from aquatic environment into mosquito larvae, (ii) assessing their persistence at different immature stages (larvae 1-4, pupae) and at the adult stage by detecting them in two mosquito organs (midgut and salivary glands), and (iii) testing the vector competence of adults exposed to AMR bacteria for dengue virus and chikungunya virus. We need to sequence the 16S gene in various samples (breeding site water, larvae and adult).
Related team publications:– Shi C, Beller L, Wang L, Rosales Rosas A, De Coninck L, Héry L, Mousson L, Pagès N, Raes J, Delang L, Vega-Rúa A, Failloux AB, Matthijnssens J. Bidirectional Interactions between Arboviruses and the Bacterial and Viral Microbiota in Aedes aegypti and Culex quinquefasciatus. mBio. 2022 Sep 7:e0102122. doi: 10.1128/mbio.01021-22.
– Shi C, Beller L, Deboutte W, Yinda KC, Delang L, Vega-Rúa A, Failloux AB, Matthijnssens J. Stable distinct core eukaryotic viromes in different mosquito species from Guadeloupe, using single mosquito viral metagenomics. Microbiome. 2019 Aug 28;7(1):121. doi: 10.1186/s40168-019-0734-2
Status: New
