Project

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#20205 : Validation of KO cell lines generated by CRISPR/Cas9 by NGS
Topics: Metagenetics (16s / 18S / ITS)
Origin: IP
Project type: Expertise

Name of Applicant: Lauriane Kergoat
Date of application: 04-09-2025
Unit: Mitochondrial Biology
Location: 66-07-04
Phone:
@ Mail: lauriane.kergoat@pasteur.fr
@ PI-Mail: timothy.wai@pasteur.fr

Project context and summary:

The aim of this project is to verify, using NGS sequencing, the KO cell lines generated in the laboratory using CRISPR/Cas9 technology.
We want to quantify the effeciency of on-target editing and identify the different mutations that may be present in a pool of edited cells.


Related team publications:
Service Delivery
Manager: marc.monot@pasteur.fr
Status: Libraries


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