Topics: Transcriptomics (Illumina)
Origin: IP
Project type: Consortium
Name of Applicant: Nadia Benaroudj
Date of application: 18-07-2024
Unit: Biology of Spirochetes
Location: 12-03-16
Phone: 3746
@ Mail: nadia.benaroudj@pasteur.fr
Project context and summary: Leptospires encompass free-living and pathogenic environmental species that are exposed to endogenous and exogenous reactive oxygen species (ROS). The adaptation to oxidative stress has been thoroughly characterized in pathogenic Leptospira species but mechanisms involved in this adaptive response are unknown in saprophytic Leptospira, given that they possess different repertoire of oxidative stress-related factors. We have identified 3 transcriptional regulators (PerRA, PerRC, OxyR) that control anti-ROS enzymes (peroxidases and SOD) in L. biflexa. Our aim is to determine the genome-wide regulon of PerRA, PerRC and OxyR.
Related team publications:Zavala-Alvarado C, Sismeiro O, Legendre R, Varet H, Bussotti G, Bayram J, G Huete S, Rey G, Coppée JY, Picardeau M, Benaroudj N. (2020) The transcriptional response of pathogenic Leptospira to peroxide reveals new defenses against infection-related oxidative stress. PLoS Pathog. Oct;16(10):e1008904 DOI: 10.1371/journal.ppat.1008904
Zavala-Alvarado C, G Huete S, Vincent AT, Sismeiro O, Legendre R, Varet H, Bussotti G, Lorioux C, Lechat P, Coppée JY, Veyrier FJ, Picardeau M, Benaroudj N. (2021) The oxidative stress response of pathogenic Leptospira is controlled by two peroxide stress regulators which putatively cooperate in controlling virulence. PLoS Pathog. Dec 2;17(12):e1009087. DOI: 10.1371/journal.ppat.1009087
G Huete S, Benaroudj N. (2023) The arsenal of Leptospira species against oxidants. Antioxidants 2023, 12(6), 1273; https://doi.org/10.3390/antiox12061273
Manager: marc.monot@pasteur.frStatus: Sequencing
