Vaccination is one of the most effective intervention to protect against infectious diseases. One of the most used vaccines is the seasonal influenza vaccine (IV) which composition is adapted every year to sample the most circulating influenza strains. However, induction of protective, neutralizing antibodies to this inactivated unadjuvanted vaccine, is variable between individuals and decreases with age. Multiple host-related factors contribute to this incomplete efficiency, and studies in humans are complicated by the heterogenous history of previous exposure to influenza virus. To identify genetic factors influencing antibody response and which may contribute to the vaccine efficiency, we immunized mice from 39 CC strains with a human tetravalent IV and analysed their antibody response against influenza H1N1 virus at several time points. CC mice showed large heritable variation in the magnitude and kinetics of antibody response, as well as in the distribution of IgG subclasses. Cytokine levels before immunization and one day after boost were also variable between strains, and random forest analysis identified patterns associated with high or low antibody responses. Now, we aim to extend these results with a global comprehensive map of the reactivity of the local immune system with the use of RNA sequencing on the lymph nodes draining the vaccination site, one day after the boost on a subset of 8 CC strains showing high, intermediate or low antibody responses .
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