Topics: Genomics (Illumina)
Origin: IP
Project type: Expertise
Name of Applicant: Basile Beaud
Date of application: 29-09-2023
Unit: Evolutionary Biology of the Microbial Cell
Location: Bertrand – 1 – 27
Phone: 0633402727
@ Mail: basile.beaud@pasteur.fr
@ PI-Mail: simonetta.gribaldo@pasteur.fr
Project context and summary: Deinococcus radiodurans is a very atypical diderm bacterium as compared to classical models like E. coli. Yet it is one of the only three models currently available to understand Terrabacteria which represent hald of bacterial diversity. We study the biogenesis of the OM of Deinococcus radiodurans which has been heavily misunderstood since it was first characterized 60 years ago. We constructed mutants of OM biogenesis genes and are going to experimentally evolve them to identify supressor mutations.
Related team publications:Megrian D, Taib N, Witwinowski J, Beloin C, Gribaldo S. One or two membranes? Diderm Firmicutes challenge the Gram-positive/Gram-negative divide. Mol Microbiol. 2020 Mar;113(3):659-671. doi: 10.1111/mmi.14469. PMID: 31975449.
Taib, N., Megrian, D., Witwinowski, J. et al. Genome-wide analysis of the Firmicutes illuminates the diderm/monoderm transition. Nat Ecol Evol 4, 1661–1672 (2020). https://doi.org/10.1038/s41559-020-01299-7
Witwinowski, J., Sartori-Rupp, A., Taib, N. et al. An ancient divide in outer membrane tethering systems in bacteria suggests a mechanism for the diderm-to-monoderm transition. Nat Microbiol 7, 411–422 (2022). https://doi.org/10.1038/s41564-022-01066-3
Manager: rania.ouazahrou@pasteur.frStatus: Data analysis
