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A cost-effective genotyping method for the major arbovirus vector, the mosquito Aedes aegypti, is lacking. We will use high-throughput sequencing of multiplexed PCR products to generate genotypes from a panel of approximately 300 carefully selected polymorphic loci. This method originally developed by Campbell et al. (2014) relies on unlabelled oligonucleotides and two rounds of PCR amplification. During this process, sequencing adapters and dual barcode sequence tags are incorporated into the amplicons enabling thousands of individuals to be pooled into a single sequencing library.
Submitted: 2023-01-31 16:38 · Last updated: 2023-01-31 16:38