Institut Pasteur
Biomics
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Phage transposon libraries

Status: Kick-off meeting

Project ID: B22030

Owner: Orlando Moranchel

Unit: Bacterial Genome Plasticity

Experiment type: To be determined

Location: Duclaux

Description

The objective is to identify essential genes in a set of bacteriophages infecting Vibrio cholerae. To do that, a transposon insertion library was produced, and Tn-positive phages enriched using an anti-CRISPR gene encoded inside the transposon. Tn librairies will also be used to screen for conditionally essential genes in chosen conditions.

Submitted: 2026-10-16 15:37 · Last updated: 2026-10-16 15:37