Status: Pending
In collaboration with the CIGM, we have generated a new knock-in mouse model by using CRISPR-CAS9 + a repair DNA matrix. We would like to know if CRISPR-CAS9 have cut in an unexpected site and if the DNA matrix has been inserted in that sites in some of these mice. The ultimated goal is to select 1 founder whithout any off-site cleavage to initiate our mouse colony.
Submitted: 2024-06-27 13:29 · Last updated: 2025-04-24 09:53