Project

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#7270 : Investigating the role of type VII secretion systems in horizontal gene transfer in mycobacteria
Topics: Genomics (Illumina)
Origin: IP
Project type: Service

Name of Applicant: Jan Madacki
Date of application: 03-06-2022
Unit: Integrated Mycobacterial Pathogenomics
Location: 61 – 02 – 08
Phone: +33 1 40 61 30 93
@ Mail: jan.madacki@pasteur.fr
@ PI-Mail: roland.brosch@pasteur.fr

Project context and summary:

Our recent findings show that tuberculosis-causing mycobacteria, similarly to related non-pathogenic mycobacteria, possess the ability to transfer chromosomal DNA from donor to recipient cells. In non-pathogenic species, the roles of two type VII secretion systems was shown to be essential for this process. In order to investigate the role of these secretion systems in tuberculosis-causing mycobacteria, we used several wild type and mutant strains in mating assays and have obtained recombinant strains. Our goal is to sequence these strains in order to identify the regions of chromosomal DNA that were transfered from the donors to the recipients.


Related team publications:
Boritsch EC, Khanna V, Pawlik A, Honoré N, Navas VH, Ma L, Bouchier C, Seemann T, Supply P, Stinear TP, Brosch R. 2016. Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria. Proc Natl Acad Sci USA 113:9876–9881.
Madacki J, Orgeur M, Mas Fiol G, Frigui W, Ma L, Brosch R. 2021. ESX-1-independent horizontal gene transfer by Mycobacterium tuberculosis complex strains. mBio 12:e00965-21.
Service Delivery
Manager: marc.monot@pasteur.fr
Status: Closed


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