We are interested in the protein regulatory variant A (RavA) that belongs to the AAA+ ATPase family and its VWA containing partner ViaA. The physiological role of RavA-ViaA (RV) complex remains unclear. Thus, we constructed a ∆ravA-viaA mutant and then evaluated the influence of RV throughout a series of growth conditions, including aerobiosis and anaerobiosis. We found that RV sensitize Escherichia coli to aminoglycosides and this effect varies with the respiratory chain used. In poor energy conservation conditions, such as in the absence of exogenous electron acceptor or under fumarate respiration, the influence of RV was very important, while in high level of energy conservation, such as in aerobiosis or under nitrate respiration, RV was dispensable. Our next objective is to investigate the physiological role of RavA-ViaA.
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