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#6643 : RNA-seq for WT vs ΔravA-viaA in anaerobiosis
Topics: Transcriptomics (Illumina)
Origin: IP
Project type: Service

Name of Applicant: Jessica El Khoury
Date of application: 20-01-2022
Unit: Department of Microbiology
Location: Biotop (12) – 5th floor
Phone: 0755812011
@ Mail: jessica.el-khoury@pasteur.fr
@ PI-Mail: frederic.barras@pasteur.fr

Project context and summary:

We are interested in the protein regulatory variant A (RavA) that belongs to the AAA+ ATPase family and its VWA containing partner ViaA. The physiological role of RavA-ViaA (RV) complex remains unclear. Thus, we constructed a ∆ravA-viaA mutant and then evaluated the influence of RV throughout a series of growth conditions, including aerobiosis and anaerobiosis. We found that RV sensitize Escherichia coli to aminoglycosides and this effect varies with the respiratory chain used. In poor energy conservation conditions, such as in the absence of exogenous electron acceptor or under fumarate respiration, the influence of RV was very important, while in high level of energy conservation, such as in aerobiosis or under nitrate respiration, RV was dispensable. Our next objective is to investigate the physiological role of RavA-ViaA.


Related team publications:
The AAA+ ATPase RavA-ViaA complex sensitizes Escherichia coli to aminoglycosides under anaerobic low energy conservation conditions Jessica Y. El Khoury, Jordi Zamarreño Beas, Allison Huguenot, Béatrice Py, Frédéric Barras bioRxiv 2022.01.13.476284; doi: https://doi.org/10.1101/2022.01.13.476284
Service Delivery
Manager: georges.haustant@pasteur.fr
Status: Closed


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