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#6094 : Function of the transcription factor pig1 in Scedosporium apiospermum.
Topics: Transcriptomics (Illumina)
Origin: Academic
Project type: Service

Name of Applicant: GASTEBOIS Amandine
Date of application: 04-10-2021
Unit: Other
Location: GEIHP UPRES EA 3142, Institut de Biologie en Santé PBH-IRIS Centre Hospitalier Universitaire 4 rue Larrey, 49 933 Angers Cedex 9
Phone: 02.44.68.83.62
@ Mail: amandine.gastebois@univ-angers.fr
@ PI-Mail: jean-philippe.bouchara@univ-angers.fr

Project context and summary:

Scedosporium apiospermum is an opportunistic filamentous fungus causing various human infections especially in immunosuppressed patients, and colonizing the respiratory tract in patients with cystic fibrosis. One research axis of the lab focuses on the adaptative mechanisms developed by this fungus in response to the particular microenvironment in CF airways. We carried out studies to identify and characterize virulence factors, and to clarify the pathogenic mechanisms of Scedosporium species.
Melanin is a component of the spore cell wall directly in contact with the lung epithelium and also with immune cells like alveolar macrophages. In S. apiospermum, the DHN-melanin biosynthesis is insured by successive enzymatic reactions, depending on the expression of a five-gene cluster, including a transcription factor, orthologue to pig1 from Magnaporthe grisea. The project objective is to decipher pig1 functions in S. apiospermum.


Related team publications:
– Pateau V, Razafimandimby J, Vandeputte P, Thornton C, Guillemette T, Bouchara JP, Giraud S. Gene Disruption of in Scedosporium aurantiacum : Proof of Concept with the Disruption of SODC Gene encoding a Cytosolic Cu,Zn – Superoxyde Dismutase. 2018. Mycopathologia 183 (1) – 241-249.
– Pihet M, Vandeputte P, Tronchin G, Renier G, Saulnier P, Georgeault S, Mallet R, Chabasse D, Symoens F, Bouchara JP. Melanin is an essential component for the integrity of the cell wall of Aspergillus fumigatus. 2008. BMC Microbiology. 24 (9), 177.
Service Delivery
Manager: marc.monot@pasteur.fr
Status: Closed


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