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#5101 : Identification of the YeiL direct regulon in E. coli
Topics: Epigenetics (ChIP-Seq)
Origin: IP
Project type: Service

Name of Applicant: Sarah Dubrac
Date of application: 05-03-2021
Unit: Other
Location: 12-05-05
Phone: 01 86 46 76 63
@ Mail: sarah.dubrac@pasteur.fr
@ PI-Mail: frederic.barras@pasteur.fr

Project context and summary:

We are studying an unknown regulator of E. coli, YeiL. It carries Fe-S cluster characteristics of proteins involved in adaptation to changing redox environment. Our transcriptomic studies have drawn a link between YeiL and anaerobic respiration of nitrate. Beyond this aspect, a lot of metabolism stress adaptation genes are regulated. We are now wondering which genes are directly regulated by YeiL to precisely define its biological role. This regulator is conserved in enterobacteria including pathogens. As anaerobic respiration in an important adaptation metabolism during gut colonization, we think that the understudied YeiL regulator could have a role for bacterial pathogenesis.
To decipher the YeiL direct regulon, we propose to use the ChIP-Seq technology. We have fused either the yeiL wild type allele or the yeiL* mutated allele, that is unable to bind the metallic center (apo-form of the regulator), to an HA-tag. We have checked that these proteins fusions were still active to regulate gene expression. Gene enrichment will be studied by comparing hits in a strain that contain the empty vector (pTet-HA), the fusion with the wild type yeiL gene, or a fusion with the modified yeiL* gene encoding the apo-form of the regulator.


Related team publications:
Vergnes, A., J. P. Viala, R. Ouadah-Tsabet, B. Pocachard, L. Loiseau, S. Meresse, F. Barras and L. Aussel, 2017. “The iron-sulfur cluster sensor IscR is a negative regulator of Spi1 type III secretion system in Salmonella enterica.” Cell Microbiol 19(4).
Mandin, P., S. Chareyre and F. Barras, 2016. “A Regulatory Circuit Composed of a Transcription Factor, IscR, and a Regulatory RNA, RyhB, Controls Fe-S Cluster Delivery.” MBio 7(5)
Chareyre, S., F. Barras and P. Mandin, 2019. “A small RNA controls bacterial sensitivity to gentamicin during iron starvation.” PLoS Genet 15(4): e1008078.
Service Delivery
Manager: juliana.pipoli-da-fonseca@pasteur.fr
Status: Closed


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