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#4079 : The effect of cold atmospheric plasma on healing of infected burn wounds (suite du 2224)
Topics: Transcriptomics (Illumina)
Origin: IP
Project type: Service

Name of Applicant: Olivier DUSSURGET
Date of application: 06-06-2020
Unit: Yersinia
Location: Duclaux, RDC haut, 02B
Phone: 0140613472
@ Mail: olivier.dussurget@pasteur.fr

Project context and summary:

Major burn injuries are life-threatening and bacterial skin infections are a leading cause of death. There are over 11 million cases of burn injuries and 300,000 burn-related deaths worldwide each year. Therefore, there is a critical need to search for new and innovative approaches to patient management and treatment.

Physical plasma is an ionized gas. Recent developments in plasma sources have made it possible to generate room-temperature plasma or cold atmospheric plasma (CAP). A large number of biochemically active compounds, predominantly reactive oxygen and nitrogen species (RONS) are generated by CAP.

Our recent studies have shown that CAP stimulated cellular migration and modulated extracellular matrix composition. Angiogenesis is a crucial component of tissue repair ensuring the formation of new blood vessels that bring nutrients and oxygen to cells within the wound. Recent in vitro and in vivo studies showed that CAP produced chemically active particles, which stimulated angiogenesis. CAP was demonstrated to have potent bactericidal properties and our in vitro and in vivo experiments showed that RONS (including hydrogen peroxide) produced by physical plasma significantly reduced bacterial growth.

The current study is aimed to further investigate antibacterial properties of CAP and identify possible mechanisms of action using a bacterial and cellular transcriptomic approach. We hope that data obtained from RNA-seq analysis will enable us to generate new hypotheses that may explain pathways involved in triggering bactericidal effects of CAP. These hypotheses will later be tested using in vitro and in vivo models, including a murine model of third-degree burn wound reconstructed with allogenic full-thickness skin graft.


Related team publications:
Cold atmospheric plasma modulates endothelial nitric oxide synthase signalling and enhances burn wound neovascularisation. Duchesne C, Banzet S, Lataillade JJ, Rousseau A, Frescaline N. J Pathol. 2019 Nov;249(3):368-380. doi: 10.1002/path.5323.
Mesenchymal Stromal Cells Based Therapy in Systemic Sclerosis: Rational and Challenges. Peltzer J, Aletti M, Frescaline N, Busson E, Lataillade JJ, Martinaud C. Front Immunol. 2018 Sep 13;9:2013. doi: 10.3389/fimmu.2018.02013.
Cell death induced on cell cultures and nude mouse skin by non-thermal, nanosecond-pulsed generated plasma. Duval A, Marinov I, Bousquet G, Gapihan G, Starikovskaia SM, Rousseau A, Janin A. PLoS One. 2013 Dec 16;8(12):e83001. doi: 10.1371/journal.pone.0083001.
Service Delivery
Manager: marc.monot@pasteur.fr
Status: Closed


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