Project

Go Back to Project List
#3003 : Understanding the transition of typical C. neoformans yeasts to Titan cells through transcriptome analysis
Topics: Transcriptomics (Illumina)
Origin: IP
Project type: Service

Name of Applicant: Alexandre Alanio
Date of application: 19-07-2019
Unit: Molecular Mycology
Location: RDC haut Duclaux aile Fourneau
Phone: 3255
@ Mail: alexandre.alanio@pasteur.fr

Project context and summary:

Cryptococcus neoformans is able to produce Titan cells which are giant (10-100 um) and polyploid cells.
These cells were mainly produced in vivo in the lung of infected mice.
However, we recently found the conditions allowing generation of Titan cells in vitro.
Together with our findings, two other different protocols have been described (in the same issue of the Journal).
We now want to take advantage of these 3 protocols to find the common baseline genes that are expressed before and during the transition towards Titan cells. We will try to understand how the transcriptome is reprogramed to undergo such drastic modification.


Related team publications:
B. Hommel, A. Sturny-Leclère, S. Volant, N. Veluppillai, M. Duchateau, C.-H. Yu, V. Hourdel, H. Varet, M. Matondo, J. R. Perfect, A. Casadevall, F. Dromer, and A. Alanio, “Cryptococcus neoformans resist to drastic conditions by switching to viable but non-culturable cell phenotype,” PLoS Pathogens, in Press
B. Hommel, L. Mukaremera, R. J. B. Cordero, C. Coelho, C. A. Desjardins, A. Sturny-Leclère, G. Janbon, J. R. Perfect, J. A. Fraser, A. Casadevall, C. A. Cuomo, F. Dromer, K. Nielsen, and A. Alanio, “Titan cells formation in Cryptococcus neoformans is finely tuned by environmental conditions and modulated by positive and negative genetic regulators,” PLoS Pathog, vol. 14, no. 5, pp. e1006982–38, May 2018.
D. Garcia-Hermoso, A. Criscuolo, S. C. Lee, M. Legrand, M. Chaouat, B. Denis, M. Lafaurie, M. Rouveau, C. Soler, J.-V. Schaal, M. Mimoun, A. Mebazaa, J. Heitman, F. Dromer, S. Brisse, S. Bretagne, and A. Alanio, “Outbreak of Invasive Wound Mucormycosis in a Burn Unit Due to Multiple Strains of Mucor circinelloides f. circinelloides Resolved by Whole-Genome Sequencing.,” mBio, vol. 9, no. 2, Apr. 2018.
Service Delivery
Manager: marc.monot@pasteur.fr
Status: Closed


Go Back to Project List