I have 50 RNA samples that I would like to perform bulk RNA seq on. The idea would be to do a regular Illumina stranded mRNA prep with sequencing on a P2 dark cycle on the NEXTSEQ2000.
I have already extracted the RNA and performed the bioanalyzer, the RINS are ok (>7). Would it be possible to get a devis for the library prep and sequencing and perhaps an estimated time as to how long it would take to get the data ?
If it’s of Use I have 50 samples,
40 samples coming from different organs across two conditions:
The two conditions are either mice transduced with a vector to overexpress a fluorescent tag GFP, which is our control and an activated condition coming from mice transduced with an excitatory protein that we have activated, and so our samples go as follow
5 olfactory bulb control vs 5 olfactory bulb activated
5 choroid plexus control vs 5 choroid plexus activated
5 hippocampus control vs 5 hippocampus activated
5 brain control vs 5 brain activated
For these the idea would be to compare only the control vs activated without going into interorgan comparisons
Then we also have 10 samples coming from control and knockout mice (5 control and 5 KO)
Let me know if this is sufficient for you
All the best
Samir
